Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fertilization01:38

Fertilization

88.5K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
88.5K
Spermatogenesis01:41

Spermatogenesis

120.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
120.5K
Spermatogenesis01:22

Spermatogenesis

8.4K
Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
8.4K
Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

8.9K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
8.9K
In Vitro Fertilization01:24

In Vitro Fertilization

773
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
773

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Superoxide Dismutase-Rich <i>Tetraselmis chuii</i> Supplementation Improves Semen Quality in Idiopathic Infertile Males: A Double-Blind Randomized Placebo-Controlled Trial.

The world journal of men's health·2026
Same author

Multiple Roles of Protamine Kinase SRPK1 and Phosphatase PP1γ in Sperm Development.

Proteomics·2026
Same author

Genetic variants through exome sequencing in Spanish patients affected by primary congenital glaucoma and juvenile open-angle glaucoma.

Molecular vision·2026
Same author

Familial interstitial lung disease: emerging insights into screening and genetic risk.

Frontiers in medicine·2026
Same author

Development of human induced pluripotent stem cell-derived ovarian support cells as a clinical-grade product for in vitro fertilization.

Cell stem cell·2026
Same author

Ovary laparoscopic incision and intraovarian platelet-rich plasma induce distinct cortical stiffness changes and follicular activation in aged sheep-a pilot study.

Journal of assisted reproduction and genetics·2026

Related Experiment Video

Updated: Dec 20, 2025

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.2K

Sperm proteomic changes associated with early embryo quality after ICSI.

Meritxell Jodar1, Claudio Attardo-Parrinello1, Ada Soler-Ventura1

  • 1EUGIN-UB Research Excellence Program; Molecular Biology of Reproduction and Development Research Group, Fundació Clínic per a la Recerca Biomèdica (FCRB), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Barcelona, Casanova 143, Barcelona 08036, Spain.

Reproductive Biomedicine Online
|May 24, 2020
PubMed
Summary

Sperm protein alterations impact embryo quality in intracytoplasmic sperm injection (ICSI) cycles. Specific sperm proteins may serve as biomarkers for predicting assisted reproductive technology outcomes.

Keywords:
Embryo qualityICSIIdiopathic infertilitySperm proteome

More Related Videos

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

539
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
03:28

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

20.5K

Related Experiment Videos

Last Updated: Dec 20, 2025

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.2K
U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

539
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
03:28

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

20.5K

Area of Science:

  • Proteomics
  • Reproductive Biology
  • Embryology

Background:

  • Sperm protein composition is increasingly recognized for its role in male fertility.
  • Understanding the sperm proteome's influence on embryo development is crucial for improving assisted reproductive technologies (ART).

Purpose of the Study:

  • To investigate whether alterations in the human sperm protein profile correlate with the quality of embryos produced via intracytoplasmic sperm injection (ICSI).
  • To identify potential sperm protein biomarkers for predicting ART success.

Main Methods:

  • Proteomic analysis of sperm samples from 27 infertile couples undergoing ICSI.
  • Tandem mass tag labeling, two-dimensional liquid chromatography, and tandem mass spectrometry for peptide identification and quantification.
  • Statistical analysis to correlate sperm protein abundance with ICSI-derived embryo quality.

Main Results:

  • The abundance of 18 individual sperm proteins correlated with ICSI embryo quality.
  • Alterations in the T-complex chaperonin were associated with poor-quality embryos.
  • Sperm proteins involved in embryogenesis, like RUBVL1, showed significant correlation with early embryo quality (r = -0.547; P = 0.028).
  • Peptide-level analysis indicated the role of post-translational modifications and isoforms.

Conclusions:

  • The sperm proteome significantly influences early embryogenesis.
  • Several sperm proteins show potential as predictive biomarkers for ART outcomes.
  • These findings may lead to improved diagnostic approaches for idiopathic infertility.