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Updated: Dec 20, 2025
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
Published on: October 21, 2022
The up-regulated hsa-circRNA9102-5 may be a risk factor for essential hypertension
Shuying Zheng1, Xin He1, Jihan Sun2
1Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medicine School of Ningbo University, Ningbo, China.
Insights
Circular RNAs (circRNAs) like hsa-circRNA9102-5 show potential as diagnostic biomarkers for essential hypertension (EH). This study found hsa-circRNA9102-5 up-regulation in EH patients, suggesting its role in EH development by interacting with hsa-miR-150-5p.
Area of Science:
- Molecular Biology
- Genetics
- Cardiovascular Research
Background:
- Essential hypertension (EH) is a prevalent cardiovascular condition with complex etiology.
- Circular RNAs (circRNAs) are emerging as critical regulators in various biological processes.
- The role of circRNAs in EH pathogenesis and their potential as biomarkers remain underexplored.
Purpose of the Study:
- To investigate the expression levels of circRNAs in the peripheral blood of EH patients and healthy controls (HC).
- To elucidate the potential role of circRNAs in EH progression.
- To evaluate circRNAs as potential diagnostic biomarkers for EH.
Main Methods:
- Microarray analysis identified differentially expressed circRNAs in peripheral blood.
- Quantitative real-time polymerase chain reaction (qRT-PCR) validated the expression of selected circRNAs in 96 EH patients and 96 HC.
- Bioinformatics predicted target microRNAs (miRNAs), and their expression was assessed via qRT-PCR.
Main Results:
- Hsa-circRNA9102-5 was significantly up-regulated in EH patients compared to HC (P = .007).
- Hsa-circRNA9102-5 demonstrated a role in EH regulation by sponging hsa-miR-150-5p.
- A diagnostic model using hsa-circRNA9102-5 achieved an AUC of 0.620, increasing to 0.728 when combined with hsa-miR-150-5p and other factors.
Conclusions:
- Hsa-circRNA9102-5 plays a crucial role in EH development, potentially via sponging hsa-miR-150-5p.
- Hsa-circRNA9102-5 represents a promising novel diagnostic biomarker and therapeutic target for essential hypertension.
Background:
The present study was aimed to investigate the expression levels of circular RNAs (circRNAs) in the peripheral blood of essential hypertension (EH) patients and healthy controls (HC). On this basis, we tried to explain the possible role of circRNAs in the progression of EH and their potential as diagnostic biomarkers of EH.
Methods:
First, we analyzed the differentially expressed circRNAs in peripheral blood obtained from the finished microarray analysis and selected four circRNAs under strict standards. Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to measure the expression levels of the selected circRNAs in a total of 192 blood samples, consisting of 96 HC and 96 diagnosed EH patients. Bioinformatics prediction of the target microRNAs (miRNAs) was performed for differentially expressed circRNAs, and the circulating vascular-related miRNAs were selected for qRT-PCR analysis to determine their expression levels.
Results:
Hsa-circRNA9102-5 (11.7 ± 1.06 vs 12.13 ± 1.11, P = .007) was up-regulated in the patients group which was diagnosed with EH, as compared to the HC group, and was involved in the regulation of EH by sponging hsa-miR-150-5p. The area under the ROC curve (AUC) of the model was 0.620, using hsa-circRNA9102-5 as an independent predictor. Furthermore, the AUC was increased to 0.728 when hsa-circRNA9102-5 was combined with hsa-miR-150-5p and multiple other factors, as a combined predictor.
Conclusions:
The present results suggested that hsa-circRNA9102-5 may have played a crucial role in the development of EH by sponging hsa-miR-150-5p, which showed great potential as a novel target.
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