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Related Experiment Video

Updated: Dec 20, 2025

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

Automated Measurement of Plasma Cell-Free Hemoglobin Using the Hemolysis Index Check Function.

Mustafa A Barbhuiya¹, Edward C Pederson¹, Monica L Straub¹

¹Department of Pathology and Laboratory Medicine, Pennsylvania State University College of Medicine, Hershey, PA.

The Journal of Applied Laboratory Medicine

|May 24, 2020

Summary

The Roche Cobas analyzer

Keywords:

Plasma cell-free hemoglobin automated testing hemolysis index

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Last Updated: Dec 20, 2025

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

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An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

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A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Area of Science:

Clinical Chemistry
Biochemical Analysis
Hemoglobin Measurement

Background:

The Roche Cobas chemistry analyzer offers a hemolysis index (HI) check function.
This function can directly report hemoglobin (Hb) concentrations.

Purpose of the Study:

To validate the HI check function for measuring plasma cell-free Hb.
To assess the analytical performance and reliability of this method.

Main Methods:

Plasma samples were analyzed using the HI check function.
Assay imprecision, lower limit of quantification (LLOQ), and analytical measurement range (AMR) were evaluated.
Accuracy was determined by comparison with a standard spectrophotometric method, alongside interference and carryover studies.

Main Results:

The assay demonstrated good precision (CVs 1.7%-7.0%) and an LLOQ of 11 mg/dL.
Excellent correlation was observed with the reference method (r=0.9990).
Significant interference was not observed for bilirubin or lipemia, and carryover was negligible.

Conclusions:

The HI check function provides a validated method for plasma cell-free Hb measurement.
This method meets analytical requirements and is both simple and cost-effective.