Renewal of conditioned tentacle lowering by circadian contextual cues in snails Cornu aspersum

Previous experiments using tentacle lowering conditioning in terrestrial snails Cornu aspersum have shown extinction and recovery of the conditioned response (CR) as a consequence of both inserting a delay between the extinction and test (spontaneous recovery) and of re-exposing the animal to the unconditioned stimulus after extinction (reinstatement). Two experiments that examined recovery of the CR due to a change in context (renewal effect) were carried out to continue this line of research. In Experiment 1, subjects received conditioning with an odour (CS) followed by extinction in the presence of another odour (CS + C), before being exposed to the original one (CS). In Experiment 2, conditioning and extinction of an odour CS took place in the presence of different circadian contextual cues (hour of the day and presence of light). The results showed that a return to the original context of conditioned training, after the extinction in a different context, either defined by an odour (Experiment 1) or by circadian cues (Experiment 2), produce a recovery of the CR compared to suitable control groups. These results can be interpreted as an instance of ABA renewal effect and they provide information about psychological mechanisms involved in extinction processes.