Salivary lubricity (ex vivo) enhances upon moderate exercise: A pilot study

This study sought to examine the effects of moderate intensity exercise on lubrication performance of saliva. We hypothesized that exercise would result in enhanced salivary lubricity by direct sympathetic stimulation of the salivary proteins.

In total, 11 healthy young pre-menopausal female participants (mean age: 24.4 ± 1.8 years, BMI: 22.1 ± 1.9 kg/m²) were included in a within-subjects repeated measures experimental design. Unstimulated whole saliva was collected at rest (S₀), immediately after 45 min of moderate intensity cycling at ∼70 % maximum heart rate (mean: 133.4 ± 0.8 bpm) or time-match quiet rest (S₁), and after a 60 min of recovery period (S₂). Ex vivo salivary lubricity were measured using soft tribology. Total protein content, mucin (MUC5B) concentration, and α-amylase activity were determined.

Tribology results revealed that moderate intensity exercise resulted in enhanced lubricity of saliva with an order-of-magnitude lower friction coefficients in the boundary regime at S₁ and S_2, with frictional forces being significantly lower at S₁ (p < 0.001) and S₂ (p < 0.001) as compared to the Control procedure. Total protein and α-amylase secretion also increased in the Exercise procedure at S₁ (p < 0.05), but concentrations returned to baseline levels at S₂.

Moderate intensity exercise leads to an increase in α-amylase and total protein secretion resulting in enhanced lubrication performance of the saliva. However, the lubrication performance was not related to MUC5B content, suggesting the role of other proteinaceous species acting as lubricants. This proof-of-concept study serves as the first step to design exercise interventions in populations with dry mouth conditions.