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The nuclear localized RIN13 induces cell death through interacting with ARF1
Xiaoxiao Liu1, Hui Liu1, Wen-Cheng Liu2
1State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Biochemical and Biophysical Research Communications
|May 25, 2020
Summary
RPM1-interacting protein 13 (RIN13) accelerates plant leaf senescence and cell death by moving Auxin Response Factor 1 (ARF1) into the nucleus. This study reveals RIN13
Area of Science:
- Plant immunity and molecular mechanisms
- Plant-pathogen interactions
- Cellular processes in plants
Background:
- RPM1-mediated resistance is crucial for plant defense against bacterial pathogens.
- RIN13 enhances RPM1-mediated resistance, but its mechanism is unclear.
- Understanding RIN13 function is key to plant disease resistance.
Purpose of the Study:
- To investigate the subcellular localization and function of RIN13 in Nicotiana benthamiana.
- To elucidate the mechanism by which RIN13 influences plant defense and senescence.
- To identify RIN13-interacting proteins and their roles.
Main Methods:
- Transient expression of RIN13 in Nicotiana benthamiana.
- Subcellular localization studies using protein fragments.
- Analysis of leaf senescence, cell death, and ROS-scavenging enzyme activity.
- Identification of RIN13-interacting proteins via co-expression.
Main Results:
- RIN13 exclusively localizes to the nucleus, with a specific region (231-300) mediating this.
- RIN13 expression accelerates leaf senescence and cell death, impacting ROS-scavenging enzymes.
- RIN13 interacts with Auxin Response Factor 1 (ARF1), which also promotes senescence.
- RIN13 facilitates ARF1 nuclear translocation.
Conclusions:
- RIN13 accelerates leaf senescence and cell death by promoting ARF1 nuclear import.
- This mechanism highlights a novel pathway in plant defense and senescence regulation.
- RIN13 plays a significant role in modulating plant responses to stress.
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