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Engineering transport systems for microbial production.

Moses Onyeabor1, Rodrigo Martinez1, Gavin Kurgan1

  • 1School of Life Sciences, Arizona State University, Tempe, AZ, United States.

Advances in Applied Microbiology
|May 25, 2020
PubMed
Summary

Metabolic engineering advances enable pathway modifications for diverse products. Optimizing microbial transport systems enhances renewable fuel and chemical production, including cellular tolerance to chemical stress.

Keywords:
Efflux pumpsMetabolic engineeringMicrobial productionProduct exportTransporters

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Area of Science:

  • Metabolic Engineering and Synthetic Biology
  • Microbial Biotechnology
  • Biochemical Engineering

Background:

  • Metabolic engineering allows complex pathway modifications for diverse product generation.
  • Optimizing substrate uptake and product export is crucial for microbial production.
  • Transport systems significantly impact the efficiency of microbial production of chemicals and fuels.

Purpose of the Study:

  • To review critical transport systems in microbial production.
  • To discuss genetic engineering strategies for improving transport functions and production metrics.
  • To highlight advancements in engineering microbial efflux systems for enhanced chemical tolerance.

Main Methods:

  • Comprehensive literature review of transport systems in metabolic engineering.
  • Analysis of genetic engineering strategies for transport optimization.
  • Examination of recent research on microbial efflux systems and stress tolerance.

Main Results:

  • Identification of key transport systems essential for microbial production.
  • Overview of genetic tools and techniques for enhancing transport capabilities.
  • Demonstration of engineered efflux systems improving cellular tolerance to chemical stress.

Conclusions:

  • Optimized transport systems are vital for efficient microbial production of fuels and chemicals.
  • Genetic engineering offers powerful strategies to enhance microbial transport functions.
  • Engineering efflux systems presents a promising avenue for increasing microbial robustness in industrial settings.