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Updated: Dec 20, 2025
Murine Skin Transplantation
Published on: January 16, 2008
Viral Vector Mediated Interleukin-10 Gene Transfer in Skin Allograft
Yeonjin Jeong1, Joseph Kyu-Hyung Park2, Seokchan Eun2
1Department of Plastic and Reconstructive Surgery, School of Medicine, Kangwon National University Hospital, Chuncheon, Korea.
Background:
Expression of genes with immunoregulatory capacity can potentially decrease rejection of allograft. According to recent studies, viral interleukin (IL)-10 can reduce immune response during allotransplantation and is one of the most promising methods for the prevention of rejection. Our study aimed to analyze the immunosuppressive potential of recombinant adenovirus-mediated rat IL-10 in rat skin allograft.
Methods:
We performed skin graft surgery 1 hour after infecting the donated skin with adenovirus-mediated rat IL-10. On day 7 postoperatively, the skin allografts were harvested, and acute rejection was graded histologically.
Results:
Viral IL-10 gene transfer into rat skin allografts improved graft survival and reduced acute rejections.
Conclusion:
The results of our study suggest that the therapeutic potential of graft viral IL-10 gene transfer is an effective immunosuppressive method for preventing skin allograft rejection.
