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Updated: Dec 20, 2025

Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts
Published on: May 16, 2021
Cardiac fibroblast activation during myocardial infarction wound healing: Fibroblast polarization after MI
Michael J Daseke1, Mavis A A Tenkorang2, Upendra Chalise2
1Department of Cellular and Integrative Physiology, Center for Heart and Vascular Research, University of Nebraska Medical Center, 985850 Nebraska Medical Center Omaha, NE 68198-5850, USA; Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS, 39216, USA; Research Service, Nebraska-Western Iowa Health Care System, Omaha, NE 68198, USA.
Abstract:
Cardiac wound healing after myocardial infarction (MI) evolves from pro-inflammatory to anti-inflammatory to reparative responses, and the cardiac fibroblast is a central player during the entire transition. The fibroblast mirrors changes seen in the left ventricle infarct by undergoing a continuum of polarization phenotypes that follow pro-inflammatory, anti-inflammatory, and pro-scar producing profiles. The development of each phenotype transition is contingent upon the MI environment into which the fibroblast enters. In this mini-review, we summarize our current knowledge regarding cardiac fibroblast activation during MI and highlight key areas where gaps remain.
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