Fluorescence quenching of various indoles by nickel complexes
Kristina Fenner1, Gustin Reynolds1, Swarna Basu1
1Department of Chemistry, Susquehanna University, 514 University Avenue, Selinsgrove, PA 17870, United States of America.
Abstract:
The interactions between two nickel complexes with the ligand N,N'-bis (2-pyridylmethylene)-1,3-diaminopropyl and the indoles melatonin, serotonin, tryptamine, and tryptophol were characterized using UV-vis and fluorescence spectroscopy. The fluorescence of all the indoles were quenched in the presence of the complex with a hydroxyl group, indicating that hydrogen-bonding is a necessary interaction for quenching to occur. Various quenching parameters were determined using Stern-Volmer analysis and the quenching was determined to be of a mixed nature with high static quenching values (1011-1013 M-1). Additional analysis using the finite sink approximation indicated that the bimolecular reactions were not diffusion-limited and had high activation energies (135-199 kJ mol-1).
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