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Biosynthesis of aromatic polyketides in microorganisms using type II polyketide synthases
Jia Wang1, Ruihua Zhang2, Xin Chen1
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029, China.
Microbial Cell Factories
|May 26, 2020
Summary
This review details aromatic polyketide biosynthesis using type II polyketide synthases (PKSs). We highlight recent advances in engineering these PKS systems to create novel molecules and enhance production.
Area of Science:
- Biochemistry and Synthetic Biology
- Natural Product Biosynthesis
Background:
- Aromatic polyketides possess significant biological and pharmacological value.
- Their unique polycyclic aromatic structures are synthesized by type II polyketide synthases (PKSs).
- Type II PKSs generate molecular diversity through sequential condensation, reduction, cyclization, aromatization, and tailoring.
Purpose of the Study:
- To review the architectural organization and genetic basis of type II PKSs.
- To summarize recent advancements in engineered biosynthesis of aromatic polyketides.
- To discuss challenges and future directions for large-scale production.
Main Methods:
- Review of literature on type II PKS architecture and genetics.
- Analysis of recent studies on engineered PKS systems for novel compound generation.
- Discussion of strategies for improving PKS-based production.
Main Results:
- Type II PKSs exhibit complex organizational and genetic features driving polyketide diversity.
- Engineering efforts have successfully produced novel aromatic polyketide structures.
- Progress has been made in enhancing product titers through PKS modification.
Conclusions:
- Understanding PKS gene contributions is key to deciphering biosynthetic pathways.
- Engineered biosynthesis offers a powerful approach to discovering and producing novel aromatic polyketides.
- Rational engineering of type II PKSs holds promise for industrial-scale natural product synthesis.
Keywords:
Aromatic polyketidesChain lengthStarter unitsTailoring reactionsType II polyketide synthasesMore Related Videos
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