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Updated: Dec 20, 2025
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
Published on: July 4, 2018
The effect of laparoscopy on mast cell degranulation and mesothelium thickness in rats
Hery Poerwosusanta1,2, Gunadi3, Zairin Noor4
1Doctoral Study Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia. herpoerwo@ulm.ac.id.
Background:
Laparoscopy induces adhesion due to ischemia-reperfusion injury. However, the detail pathomechanism is poorly understood. This study aimed to investigate the impact of laparoscopy on mast cell and mesothelium morphological changes in the rat.
Methods:
Forty-nine males of Sprague-Dawley Rattus norvegicus were divided into four groups: a) control and b) intervention groups P1, P2, and P3 that underwent 60 min laparoscopic using carbon dioxide (CO2) insufflation at 8, 10, and 12 mmHg groups, respectively. Serum hydrogen peroxide (H2O2), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and oxidative stress index (OSI) levels were determined 24 h after laparoscopy. Histopathological analyses of mast cell infiltration and degranulation and mesothelium thickness in the liver, greater omentum, mesenterium, small intestine, and peritoneum were performed 7 days after the procedure.
Results:
H2O2, MDA, and OSI levels were significantly increased in the intervention groups compared with the control (p<0.05), while the SOD and CAT levels were decreased in the intervention groups compared with the control (p<0.05). Mast cell infiltration and degranulation were higher in the intervention groups than in control (p<0.05), while the mesothelium thickness was significantly lower in the laparoscopic groups than in control (p<0.05). Interestingly, the decrease in mesothelium thickness was strongly associated with the increase in mast cell infiltration and degranulation (p<0.01).
Conclusions:
Our study shows that laparoscopy in rats increases mast cell infiltration and degranulation, which also results in and correlates with a decrease in mesothelial thickness.
Insights
Laparoscopy increases mast cell activation and decreases mesothelial thickness in rats. This suggests a link between mast cells and tissue damage following laparoscopic procedures.
Area of Science:
- Surgical Pathology
- Inflammatory Response
- Tissue Engineering
Background:
- Laparoscopy can lead to adhesions, potentially via ischemia-reperfusion injury.
- The precise mechanisms underlying laparoscopy-induced adhesions are not fully understood.
- Investigating cellular and morphological changes is crucial for understanding surgical complications.
Purpose of the Study:
- To examine the effects of laparoscopy on mast cell behavior.
- To assess morphological changes in the mesothelium after laparoscopy.
- To elucidate the pathomechanism of laparoscopy-induced tissue injury in rats.
Main Methods:
- Rats underwent 60-minute laparoscopy with varying carbon dioxide insufflation pressures.
- Serum markers of oxidative stress (H2O2, MDA, OSI, CAT, SOD) were measured.
- Histopathological analysis evaluated mast cell infiltration/degranulation and mesothelium thickness in multiple organs.
Main Results:
- Laparoscopy significantly increased oxidative stress markers (H2O2, MDA, OSI) and decreased antioxidant enzymes (CAT, SOD).
- Increased mast cell infiltration and degranulation were observed in laparoscopy groups.
- Mesothelium thickness significantly decreased, correlating strongly with mast cell activation.
Conclusions:
- Laparoscopic procedures in rats induce significant mast cell degranulation and infiltration.
- A notable decrease in mesothelial thickness occurs post-laparoscopy.
- Mast cell activation is closely associated with reduced mesothelial thickness following laparoscopy.
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