Related Experiment Video

Updated: Dec 20, 2025

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

8.5K

Intracellular functions of RNA-binding protein, Musashi1, in stem and cancer cells

Mahboobeh Forouzanfar1, Liana Lachinani2, Kianoush Dormiani3

  • 1Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar Jerib Ave., Azadi Square, Isfahan, P.O. Code 81746, Iran.

Stem Cell Research & Therapy
|May 26, 2020
PubMed

Insights

Musashi1 (MSI1) protein drives stem cell proliferation and cancer development by activating key signaling pathways. Inhibiting MSI1 shows promise for cancer therapy by reducing tumor growth and metastasis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Developmental Biology

Background:

  • Musashi1 (MSI1) is an RNA-binding protein crucial for asymmetric cell division in sensory organ precursors.
  • MSI1 expression is frequently upregulated in various cancers, correlating with stem cell proliferation and cancer progression.

Purpose of the Study:

  • To review the multifaceted role of MSI1 in stem cell proliferation and cancer promotion.
  • To explore MSI1's involvement in critical signaling pathways like Wnt, Notch, and Akt in cancer.

Main Methods:

  • Literature review of studies investigating MSI1 function in stem cells and cancer.
  • Analysis of MSI1's regulatory mechanisms, including microRNA-mediated control.
  • Examination of MSI1's impact on cancer stem cell proliferation, malignancy, and chemoresistance.

Main Results:

  • MSI1 is essential for stem and cancer stem cell proliferation, acting via Wnt, Notch, and Akt signaling pathways.
  • MSI1 upregulation promotes cancer malignancy, chemoresistance, and tumor growth.
  • Tumor suppressor microRNAs negatively regulate MSI1, inhibiting cancer cell migration and metastasis.

Conclusions:

  • MSI1 is a significant driver of cancer development and metastasis, making it a potential therapeutic target.
  • Targeting MSI1 offers a promising strategy to inhibit cancer stem cell proliferation and reduce tumor burden.
Keywords:
Cancer progressionCancer stem cellsMusashi, RNA-binding protein

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.3K
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

28.0K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.7K