microRNA-1271 impedes the development of prostate cancer by downregulating PES1 and upregulating ERβ

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Updated: Dec 20, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.9K

Zhenming Jiang1, Yuxi Zhang2,3, Xi Chen4

  • 1Department of Urology, The First Hospital of China Medical University, No. 155 Nanjing North Street, Heping District, Shenyang, 110001, Liaoning Province, People's Republic of China.

Journal of Translational Medicine
|May 26, 2020
PubMed
Abstract

Insights

Overexpression of microRNA-1271 (miR-1271) downregulates pescadillo homolog 1 (PES1), activating the ERβ pathway and delaying prostate cancer development. miR-1271 shows potential as a novel prostate cancer treatment biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Pescadillo homolog 1 (PES1) is a nucleolar protein involved in ribosome biogenesis and implicated in various cancers.
  • The specific role and mechanism of PES1 in prostate cancer progression remain unclear.
  • This study investigates the function and molecular pathways of PES1 in prostate cancer.

Purpose of the Study:

  • To elucidate the role of PES1 in prostate cancer.
  • To identify the regulatory mechanism involving PES1 and microRNA-1271 (miR-1271) in prostate cancer.
  • To explore the therapeutic potential of targeting PES1 or miR-1271 in prostate cancer.

Main Methods:

  • Differential gene expression analysis identified PES1 as upregulated in prostate cancer.
  • Bioinformatics and dual-luciferase assays confirmed the interaction between PES1 and miR-1271.
  • RT-qPCR, cell-based assays (EdU, flow cytometry, Transwell), and a mouse model assessed the functional impact of miR-1271 and PES1.

Main Results:

  • PES1 was upregulated in prostate cancer tissues and cells.
  • miR-1271 was downregulated in prostate cancer and directly targets PES1.
  • Overexpression of miR-1271 or PES1 depletion inhibited proliferation, migration, and invasion, while promoting apoptosis and suppressing tumor growth in vivo.

Conclusions:

  • miR-1271 functions as a tumor suppressor in prostate cancer by downregulating PES1 and activating the ERβ signaling pathway.
  • This miR-1271/PES1/ERβ axis offers a novel therapeutic strategy for prostate cancer.
  • miR-1271 demonstrates potential as a diagnostic and therapeutic biomarker for prostate cancer.
Keywords:
Estrogen receptor βPescadillo homolog 1Prostate cancermicroRNA-1271

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