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Published on: July 9, 2014
RT-qPCR for the diagnosis of the vesiculovirus Cocal virus
Mariana Lázaro Sales1, Mariana Dall'Agnol2, Anapolino Macedo de Oliveira3
1Departamento de Medicina Veterinária Preventiva da Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Abstract:
Cocal virus (COCV) is one of the causative agents of vesicular stomatitis, presenting clinical signs indistinguishable from those caused by foot-and-mouth disease virus (FMDV). Therefore, the differentiation of these two viruses via laboratory diagnosis is essential. The objective of this study was to develop and validate a real-time quantitative PCR (RT-qPCR) protocol for the diagnosis of COCV directly from epithelial samples. The method developed had 97% accuracy at 3950 pfu and a repeatability error of 1.29%. RT-qPCR was able to distinguish COCV from other viruses that cause vesicular diseases, an important factor because seroneutralization may produce cross-reactivity between COCV and vesicular stomatitis Alagoas virus (VSAV). No epithelial sample originating from vesicular disease outbreaks between 2014 and 2018 in Brazil was positive for COCV.
