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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii

Mengjun Cheng1, Man Luo2, Hengyu Xi2

  • 1Shanghai Institute of Phage, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.

Virus Genes
|May 26, 2020
PubMed

Insights

A novel lytic bacteriophage, vB_ApiP_XC38, was isolated from the multidrug-resistant Acinetobacter pittii pathogen. Genomic and phylogenetic analyses suggest this phage represents a new species within the Podoviridae family.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Acinetobacter pittii is a significant cause of hospital-acquired infections globally.
  • Multidrug resistance in A. pittii necessitates alternative therapeutic strategies.

Purpose of the Study:

  • To isolate and characterize a novel lytic bacteriophage targeting multidrug-resistant A. pittii.
  • To perform comprehensive genomic and phylogenetic analyses of the isolated phage.

Main Methods:

  • Isolation of bacteriophage vB_ApiP_XC38 using A. pittii ABC38 as the host.
  • Sequencing and bioinformatic analysis of the phage genome.
  • Morphological, genomic, and phylogenetic comparative analyses.

Main Results:

  • The lytic phage vB_ApiP_XC38, belonging to the Podoviridae family, was successfully isolated.
  • The phage possesses a double-stranded genome (79,328 bp) with 97 predicted open reading frames.
  • Genomic analysis revealed low similarity to known phages and bacteria, with distinct modules for nucleotide metabolism, replication, structure, and lysis.

Conclusions:

  • Morphological, genomic, and phylogenetic data strongly support vB_ApiP_XC38 as a novel phage species infecting A. pittii.
  • This discovery offers potential for phage therapy against multidrug-resistant A. pittii infections.
Keywords:
Acinetobacter pittiiGenome analysisOpportunistic pathogenPhage

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