Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SAFE: a Mix-and-Read Assay for miRNA Detection in Extracellular Vesicles From Unprocessed Plasma Toward Clinical Disease Diagnosis.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Plasmonic Nanomachines: Creating Local Potential Gradients and Motions.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Correction to "Precisely Shaped, Uniformly Formed Gold Nanocubes with Ultrahigh Reproducibility in Single-Particle Scattering and Surface-Enhanced Raman Scattering".

Nano letters·2026
Same author

3D mapping of compositional gradients of core-shell structures in AgIn<sub>x</sub>Ga<sub>1-x</sub>S<sub>2</sub> quantum dots by atom probe tomography.

Nature communications·2026
Same author

Plasmon Mode-Selective Gold Nanodimers with a Metal-Semiconductor Hybrid Junction.

ACS nano·2026
Same author

Metal-Organic Framework/Au Core/Shell Nanocubes for Spatially Aligned Molecular Analysis and Molecular-Fingerprint Sensing with Surface-Enhanced Raman Scattering.

Journal of the American Chemical Society·2025
Same journal

Design Principles for Fluid Molecular Ferroelectrics.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Generating Unconventional Spin-Orbit Torques With Patterned Phase Gradients in Tungsten Thin Films.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

An In Situ H<sub>2</sub>S-Activated Plasmonic Nanozyme for Near-Infrared II Photo-Thermoelectric Catalytic Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

A Recyclable and Sustainable Hydroxypropyl Methylcellulose Electrolyte for Electrochromic Devices.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Perovskite Heterostructures for Optoelectronic Applications.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Light-Written Nonvolatile Polarization via Defect-Engineered Charge Trapping.

Advanced materials (Deerfield Beach, Fla.)·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.9K

A Lipid-Nanopillar-Array-Based Immunosorbent Assay.

Jieun Kim1, Sungi Kim1, Junhyoung Ahn2

  • 1Department of Chemistry, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.

Advanced Materials (Deerfield Beach, Fla.)
|May 26, 2020
PubMed
Summary
This summary is machine-generated.

A new lipid-nanopillar-array-based immunosorbent assay (LNAIA) offers rapid, sensitive virus detection. This advanced platform surpasses traditional ELISA for faster, more accurate viral quantification.

Keywords:
biosensorsimmunosorbent assayslipid nanopillar arrayssupported lipid bilayersvirus detection

More Related Videos

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.5K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.7K

Related Experiment Videos

Last Updated: Dec 20, 2025

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.9K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.5K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.7K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Immunology

Background:

  • Viral infections pose significant global health and economic threats.
  • Conventional Enzyme-Linked Immunosorbent Assay (ELISA) for virus detection has limitations in sensitivity, quantification range, and speed.
  • A need exists for improved platforms for rapid, sensitive, and selective virus detection.

Purpose of the Study:

  • To develop a novel lipid-nanopillar-array-based immunosorbent assay (LNAIA) for enhanced virus detection.
  • To improve upon the sensitivity, speed, and quantification capabilities of existing viral detection methods.
  • To enable rapid and quantitative analysis of viral binding events.

Main Methods:

  • Development of a substrate featuring a nanopillar-supported lipid bilayer.
  • Immobilization of fluorophore-modified antibodies for virus detection.
  • Utilizing 3D nanopillar array structures and fluid antibodies for efficient target binding.
  • Employing conventional fluorescence microscopy for analysis.

Main Results:

  • The LNAIA platform demonstrated rapid and sensitive detection of viruses.
  • Achieved quantification of H1N1 virus down to 150 particles.
  • Exhibited a 5-orders-of-magnitude dynamic range within 25 minutes.
  • Showcased superior performance compared to conventional ELISA platforms.

Conclusions:

  • LNAIA offers a significant advancement in virus detection technology.
  • The platform provides rapid, sensitive, and quantitative viral analysis.
  • LNAIA overcomes key limitations of traditional ELISA, enabling faster and more precise diagnostics.