Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AgRP neuron activity predicts and tracks the glycemic response to oral glucose.

Molecular metabolism·2026

Same author

AgRP Neuron Activity Predicts and Tracks the Glycemic Response to Oral Glucose.

bioRxiv : the preprint server for biology·2026

Same author

Understanding Clinical Reasoning Variability in Medical Large Language Models: A Mechanistic Interpretability Study.

medRxiv : the preprint server for health sciences·2026

Same author

Multi-omics analysis reveals a unique epigenetic signature in <i>MYD88</i> wild-type Waldenström macroglobulinemia.

Haematologica·2025

Same author

Deleterious Effects of a GAD65 Monoclonal Autoantibody on Islet Function.

Diabetes·2025

Same author

A consensus guide to preclinical indirect calorimetry experiments.

Nature metabolism·2025

Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026

Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026

Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026

Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026

Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026

Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 20, 2025

Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques

Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques

Published on: May 7, 2020

Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques.

Chelsea L Faber¹, Miles E Matsen¹, Thomas H Meek¹

¹Department of Medicine, University of Washington.

Journal of Visualized Experiments : Jove

|May 26, 2020

Summary

This summary is machine-generated.

This study presents a novel angled coronal approach for precise stereotactic targeting in rodent brains, crucial for advanced neuroscience techniques. The method successfully targeted the ventromedial nucleus (VMN) to reveal its role in glucose regulation.

More Related Videos

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

Published on: July 16, 2020

Magnetic Resonance-Guided Stereotaxy for Infusions to the Pig Brain

Magnetic Resonance-Guided Stereotaxy for Infusions to the Pig Brain

Published on: March 31, 2023

Related Experiment Videos

Last Updated: Dec 20, 2025

Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques

Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques

Published on: May 7, 2020

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

Published on: July 16, 2020

Magnetic Resonance-Guided Stereotaxy for Infusions to the Pig Brain

Magnetic Resonance-Guided Stereotaxy for Infusions to the Pig Brain

Published on: March 31, 2023

Area of Science:

Neuroscience
Stereotactic Surgery
Rodent Models

Background:

Stereotactic surgery is vital in neuroscience but faces challenges in targeting midline brain structures and accommodating advanced technique hardware.
Accurate targeting of discrete brain nuclei, like the ventromedial nucleus (VMN), is difficult due to anatomical constraints.

Purpose of the Study:

To present a modifiable angled coronal approach for stereotactic targeting in rodent brains.
To demonstrate the protocol's adaptability for various neuroscience techniques and brain regions.
To target the mouse VMN for optogenetic experiments investigating glucose counterregulation.

Main Methods:

Developed a modifiable angled coronal stereotactic targeting protocol for mice and rats.
Utilized adeno-associated virus (AAV) for gene delivery of SwiChR++ to Cre-dependent mouse models.
Performed angled bilateral implantation of fiberoptic cannulae into the VMN.

Main Results:

The angled coronal approach enabled precise targeting of the mouse VMN.
Activation of a subset of VMN neurons was shown to be essential for glucose counterregulatory responses.
The study demonstrated the protocol's utility in optogenetic inhibition experiments.

Conclusions:

The angled coronal approach offers a versatile solution for stereotactic targeting in neuroscience research.
This method facilitates the integration of advanced techniques like optogenetics by overcoming spatial limitations.
Targeting the VMN is critical for understanding glucose homeostasis and its regulation.