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Updated: Dec 20, 2025

Potato Virus X-Based microRNA Silencing VbMS In Potato.
Published on: May 11, 2020
Potato Virus X-Based microRNA Silencing (VbMS) In Potato
Jinping Zhao1, Carlos Garcia Rios1, Junqi Song2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System.
Abstract:
Virus-based microRNA silencing (VbMS) is a rapid and efficient tool for functional characterization of microRNAs (miRNAs) in plants. The VbMS system has been developed and applied for various plant species including Nicotiana benthamiana, tomato, Arabidopsis, cotton, and monocot plants such as wheat and maize. Here, we describe a detailed protocol using PVX-based VbMS vectors to silence endogenous miRNAs in potato. To knock down the expression of a specific miRNA, target mimic (TM) molecules of miRNA of interest are designed, integrated into plant virus vectors, and expressed in potato by Agrobacterium infiltration to bind directly to the endogenous miRNA of interest and block its function.
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