Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TP53 isoform dysregulation in pediatric B-ALL: identifying markers of favorable prognosis and relapse-associated dynamic.

Molecular biology reports·2026
Same author

Diagnostic and prognostic utility of neutrophil CD64 and monocyte HLA-DR in adult sepsis patients.

BMC research notes·2026
Same author

Bone marrow mesenchymal stromal cells and multiple myeloma: Cellular interactions and therapeutic opportunities.

Pathology, research and practice·2026
Same author

Molecular Signatures of Pyomelanin Production in MDR <i>Acinetobacter baumannii</i>: A Proteomic Perspective.

Journal of proteome research·2026
Same author

Combining conventional hemogram and reticulocyte metrics enhances iron deficiency detection in asymptomatic individuals.

Clinica chimica acta; international journal of clinical chemistry·2026
Same author

Comparative Analysis of 12 Flow Cytometry-Based Markers in B-Lymphoblastic Leukemia/Lymphoma and Their Utility in Detecting Minimal/Measurable Residual Disease.

International journal of laboratory hematology·2025
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

21.4K

A flow cytometric cell-cycle assay using methyl green.

Pooja Murgai1, Prashant Sharma1, Man Updesh Singh Sachdeva1

  • 1Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Analytical Biochemistry
|May 26, 2020
PubMed
Summary
This summary is machine-generated.

Methyl green (MG) offers a new, affordable flow cytometry assay for cell-cycle and DNA-ploidy analysis. This accurate method provides a cost-effective alternative to traditional propidium iodide (PI) DNA-ploidy assays.

Keywords:
Cell cycleDNA-IndexFlow cytometryFluorescent dyeMethyl greenS-phase fraction

More Related Videos

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

61.1K
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

19.9K

Related Experiment Videos

Last Updated: Dec 20, 2025

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

21.4K
Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

61.1K
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

19.9K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Flow cytometry is crucial for cell-cycle analysis and DNA ploidy assessment.
  • Conventional assays often rely on expensive or hazardous reagents.
  • Methyl green (MG) is a low-cost, established histological stain with DNA-binding properties.

Purpose of the Study:

  • To develop and validate a novel flow cytometric assay for cell-cycle and DNA-ploidy analysis using Methyl green (MG).
  • To evaluate the performance and cost-effectiveness of the MG-based assay compared to existing methods.

Main Methods:

  • A lyse-permeabilize-stain protocol was optimized for MG staining.
  • Fluorometry was used to characterize MG's spectral properties and DNA binding.
  • Assay linearity, precision (coefficient of variation of G0/G1 peaks), and carryover were assessed.
  • The MG assay was validated against a propidium iodide (PI)-based kit using acute lymphoblastic leukemia (ALL) specimens.

Main Results:

  • MG demonstrated optimal excitation/emission characteristics for flow cytometry when bound to DNA.
  • The MG assay showed linear DNA binding, low coefficients of variation for G0/G1 peaks, and minimal carryover.
  • High correlation was observed between the MG-based assay and the PI-based kit in 29 ALL specimens.

Conclusions:

  • Methyl green (MG) can be effectively utilized to create a robust flow cytometric DNA-ploidy assay.
  • The MG-based assay is an accurate, inexpensive, and viable alternative to conventional PI-based DNA-ploidy assays.