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Urinary LDH isoenzyme 5 excretion in experimental pyelonephritis
British Journal of Experimental Pathology
|April 1, 1977
Summary
Kidney infection from Escherichia coli significantly increased urinary lactic dehydrogenase 5 (ULDH 5) excretion in rats. This finding correlated with higher white blood cell excretion, indicating a potential marker for infection.
Area of Science:
- Nephrology
- Biochemistry
- Microbiology
Background:
- Urinary lactic dehydrogenase (ULDH) isoenzymes are potential biomarkers for kidney disease.
- Escherichia coli pyelonephritis is a common kidney infection requiring diagnostic markers.
Purpose of the Study:
- To investigate the impact of kidney parenchymal infection on ULDH isoenzyme composition.
- To assess ULDH 5 as a potential indicator of pyelonephritis.
Main Methods:
- Experimental pyelonephritis was induced in Sprague-Dawley rats using Escherichia coli.
- Urine samples were collected over 10 days to analyze ULDH isoenzymes and osmolality.
- Leukocyte excretion rates were measured and correlated with ULDH 5 activity.
Main Results:
- Infected rats exhibited significantly lower urine osmolality compared to sham controls.
- Elevated ULDH 5 excretion was observed in the experimental group throughout the 10-day study.
- A strong positive correlation (r=0.815) was found between ULDH 5 activity and leukocyte excretion.
Conclusions:
- ULDH 5 excretion is significantly elevated during experimental Escherichia coli pyelonephritis in rats.
- Increased ULDH 5 levels correlate with leukocyte excretion, suggesting its potential as a biomarker for kidney infection.
- Further research is needed to confirm causality and clinical applicability.