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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Modern Molecular Taxonomy01:29

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Updated: Dec 20, 2025

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects.

David Ballard1, Jakub Winkler-Galicki2, Joanna Wesoły3

  • 1King's Forensic Genetics, Faculty of Life Sciences and Medicine, King's College London, 150 Stamford Street, London, UK.

International Journal of Legal Medicine
|May 27, 2020
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers significant advantages for forensic science, overcoming initial challenges. This technology is revolutionizing forensic investigations with its advanced capabilities.

Keywords:
MPSNGSNanopore sequencingNucleic acidsSequencing by synthesis

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Area of Science:

  • Forensic Science
  • Genomics
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS), also known as massive parallel sequencing (MPS), has become a transformative technology in biological research over the past decade.
  • Its adoption in forensics was initially hindered by a lack of accredited equipment, specialized kits, and higher error rates compared to Sanger sequencing.

Purpose of the Study:

  • To discuss the utility and application of NGS technology in the field of forensic science.
  • To highlight the benefits and challenges associated with implementing NGS in forensic casework.
  • To explore the technical aspects, commercial availability, and future potential of MPS in forensics.

Main Methods:

  • Review of current literature and technical reports on NGS applications in forensics.
  • Analysis of advantages and disadvantages of NGS compared to traditional methods like Sanger sequencing.
  • Discussion of experimental considerations and commercially available NGS solutions for forensic use.

Main Results:

  • NGS technology has largely overcome earlier limitations, including accreditation and error rates, making it viable for forensic applications.
  • Numerous reports demonstrate the successful implementation and benefits of NGS in various forensic contexts.
  • Commercial solutions and refined methodologies are increasingly available, supporting forensic adoption.

Conclusions:

  • NGS technology presents a powerful tool for forensic investigations, offering enhanced capabilities beyond traditional methods.
  • The continued development and validation of NGS platforms are crucial for its widespread acceptance and application in forensic science.
  • Future applications of MPS in forensics hold significant promise for improving identification and investigative processes.