Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

120.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
120.5K
Spermatogenesis01:22

Spermatogenesis

8.4K
Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of CYP3A5 and CYP3A4 in the metabolism and toxicity of 10-hydroxyl mesaconitine, a new potential toxicity marker of Radix Aconiti Lateralis Preparata (Fuzi), in vitro and in vivo.

Drug metabolism and disposition: the biological fate of chemicals·2026
Same author

HPV16E7-Specific Affitoxin Induces GSDME-Mediated Pyroptosis via a Caspase-3-Independent Pathway.

International journal of antimicrobial agents·2026
Same author

Frequency-domain stability analysis of mixed traffic flow considering communication degradation and human driving heterogeneity.

PloS one·2026
Same author

RBF++: Quantifying and Optimizing Reasoning Boundaries across Measurable and Unmeasurable Capabilities for Chain-of-Thought Reasoning.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

First-principles prediction of β-phase SnA<sub>2</sub>N<sub>4</sub> (A = Si; Ge) monolayers: outstanding mechanical anisotropy and high electron mobility for FET devices.

Physical chemistry chemical physics : PCCP·2026
Same author

Advances in artificial metabzymes for molecular metabolism restoration in aging-related diseases.

Chemical communications (Cambridge, England)·2026

Related Experiment Video

Updated: Dec 20, 2025

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

2.1K

CdSe/ZnS quantum dots induced spermatogenesis dysfunction via autophagy activation.

Qingling Yang1, Fangyuan Li1, Yanyan Miao2

  • 1Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Henan Provincial Obstetrical and Gynecological Diseases (Reproductive Medicine) Clinical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Henan Engineering Laboratory of Preimplantation Genetic Diagnosis and Screening, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Journal of Hazardous Materials
|May 27, 2020
PubMed
Summary

Cadmium Selenide/Zinc Sulfide quantum dots (QDs) harm sperm production by disrupting DNA repair in spermatocytes. Inhibiting autophagy, a cellular process, can restore sperm production and reduce nanoparticle toxicity.

Keywords:
AutophagyCdSe/ZnS quantum dotsDouble-strand break repairSpermatogenesisTestis

More Related Videos

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.6K
Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications
10:56

Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications

Published on: February 6, 2016

14.4K

Related Experiment Videos

Last Updated: Dec 20, 2025

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

2.1K
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.6K
Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications
10:56

Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications

Published on: February 6, 2016

14.4K

Area of Science:

  • Reproductive Toxicology
  • Nanomedicine
  • Molecular Biology

Background:

  • Nanoparticles pose risks to male reproductive health, but mechanisms remain unclear.
  • Previous studies focused on sperm parameters, neglecting detailed molecular pathways.
  • Effective strategies to mitigate nanoparticle-induced testicular toxicity are needed.

Purpose of the Study:

  • To elucidate the mechanism of CdSe/ZnS quantum dots (QDs) toxicity on spermatogenesis.
  • To investigate the role of autophagy in nanoparticle-induced testicular damage.
  • To evaluate autophagy inhibition as a therapeutic strategy against male reproductive toxicity.

Main Methods:

  • Exposure of spermatocytes to CdSe/ZnS QDs in vitro and in vivo.
  • Assessment of DNA double-strand break (DSB) repair and meiotic progression.
  • Analysis of autophagy levels and gene expression related to homologous recombination.
  • Administration of autophagy inhibitor 3-methyladenine (3-MA) to assess protective effects.

Main Results:

  • CdSe/ZnS QDs exposure impaired DSB repair in spermatocytes, disrupting meiosis and causing apoptosis.
  • QDs exposure led to elevated autophagy, which downregulated homologous recombination gene expression.
  • Inhibition of autophagy using 3-MA restored DSB repair, prevented apoptosis, and recovered sperm production.
  • Autophagy was identified as a key mediator of nanoparticle-induced spermatogenesis dysfunction.

Conclusions:

  • Autophagy plays a critical role in mediating testicular toxicity induced by CdSe/ZnS QDs.
  • Autophagy inhibition is a promising therapeutic approach to counteract nanoparticle-induced male reproductive toxicity.