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Published on: December 15, 2015
Development and Validation of a High-performance Liquid Chromatography-Diode-array Detection Method for the
Ebru Türköz Acar1, Mehmet Engin Celep2, Mohammad Charehsaz3
1Yeditepe University, Faculty of Pharmacy, Department of Analytical Chemistry, İstanbul, Turkey.
Objectives:
A new HPLC method was developed and validated for the determination of some phenolic compounds; gallic acid, chlorogenic acid, epigallocatechin, caffeic acid, vanillin, p-coumaric acid, rutin, and quercetin in some local wine and fruit wine samples.
Materials And Methods:
Analyses were performed on a Zorbax Eclipse C18 column (4.6 x 150 mm, 3.5-µm particle size) using a gradient system. Mobile phase A was a 10-mM phosphoric acid solution and mobile phase B was methanol using a flow rate of 1 mL/min. Phenolic components were monitored using a DAD at three different wavelengths.
Results:
The developed and validated method was generally linear between the 1-100 ppm concentration range. Recovery values were obtained in the range of 95-105% and repetitive. The method was successfully applied to investigate the phenolic profiles of different wine samples.
Conclusion:
As a result of the study, an accurate, sensitive and reliable HPLC-DAD method was developed. The method was successfully used to determine the concentrations of antioxidant phenolic constituents from some local wine extracts.
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