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Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of Artemisia campestris from
Boulanouar Bakchiche1, Abdelaziz Gherib1, Maria Rosário Bronze2
1Amar Telidji University, Faculty of Technology, Laboratory of Process Engineering, Laghouat, Algeria.
Objectives:
Our study aimed to investigate the chemical profile of hydroalcoholic extract of Algerian Artemisia campestris and its antioxidant activity.
Materials And Methods:
The hydroalcoholic extract of Algerian A. campestris was investigated for its phenolic constituents using high performance liquid chromatography (HPLC)-diode array detection (DAD)-electrospray ionization (ESI)-mass spectrometer (MS)/MS. The in vitro antioxidant activity and total phenolic content were also evaluated via oxygen radical absorbance capacity and Folin-Ciocalteu assays, respectively.
Results:
HPLC-DAD-ESI-MS/MS analysis revealed that the main tentatively identified compounds were caffeoylquinic acid isomers, flavonoids, and benzoic acid derivatives. Additionally, the hydroalcoholic extract exhibited a promising antioxidant activity value of 120.5±10.4 μmol Trolox equivalent antioxidant capacity/g dry weight (DW), and a strong correlation exists between this activity and the total phenolic content value of 102.09±1.65 mg/g gallic acid equivalents DW.
Conclusion:
The hydroalcoholic extract of A. campestris is a promising candidate for the production of naturally occurring antioxidant agents.
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