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Separation of neutral reducing oligosaccharides derived from glycoproteins by HPLC on a hydroxylated polymeric
B Bendiak1, J Orr, I Brockhausen
1Department of Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
Analytical Biochemistry
|November 15, 1988
Abstract:
A prototype hydroxylated polymeric support, Glyco-PAK N, was employed to separate a number of neutral reducing oligosaccharides derived from various glycoproteins by the hydrazinolysis/re-N-acetylation/mild acid hydrolysis procedure. General features of the separation properties of the matrix are described. A major advantage of the packing over silica-based supports is its stability, a property which is advantageous both in repetitive analytical chromatography and in the preparative isolation and spectroscopic identification of pure compounds, free of column breakdown products.