Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

18.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.5K
CRISPR01:59

CRISPR

57.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.4K
Homologous Recombination02:31

Homologous Recombination

61.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
61.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CAG-targeting artificial miRNA with reduced off-target risk for efficient lowering of pathogenic polyglutamine proteins.

NAR molecular medicine·2026
Same author

Challenges in visualizing endogenous <i>loci</i> in the human genome using CRISPR-based imaging systems.

Biotechnologia·2026
Same author

CRISPR/Cas9-induced double-strand breaks in the huntingtin locus lead to CAG repeat contraction through DNA end resection and homology-mediated repair.

BMC biology·2024
Same author

In Vitro Evidence of Selective Pro-Apoptotic Action of the Pure Cannabidiol and Cannabidiol-Rich Extract.

Molecules (Basel, Switzerland)·2023
Same author

Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture.

Life (Basel, Switzerland)·2023
Same author

Advances in Modeling Polyglutamine Diseases Using Genome Editing Tools.

Cells·2022

Related Experiment Video

Updated: Dec 20, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.2K

Computational Tools and Resources Supporting CRISPR-Cas Experiments.

Pawel Sledzinski1, Mateusz Nowaczyk1, Marta Olejniczak1

  • 1Department of Genome Engineering, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.

Cells
|May 28, 2020
PubMed
Summary

CRISPR-Cas technology enables precise genome engineering. This review covers computational tools for guide RNA design, predicting editing outcomes, and analyzing sequencing data to advance CRISPR-Cas experiments.

Keywords:
CRISPRHDRNGS analysisNHEJdatabasegenome editingmicrohomologyoff-targetpredictive algorithmssgRNAsgRNA design

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.7K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.4K

Related Experiment Videos

Last Updated: Dec 20, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.2K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.7K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.4K

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • CRISPR-Cas systems, particularly using Cas9 nuclease, are transformative for targeted genome engineering.
  • The field's rapid advancement necessitates sophisticated computational tools for experimental design and analysis.
  • Numerous bioinformatics tools have emerged to support various stages of CRISPR-Cas applications.

Purpose of the Study:

  • To provide a comprehensive and updated overview of computational tools for CRISPR-Cas genome editing.
  • To guide users in selecting reliable tools for different experimental steps, from guide RNA design to outcome analysis.
  • To discuss current limitations and future challenges in CRISPR-Cas computational tool development.

Main Methods:

  • Review and categorization of existing CRISPR-Cas related computational tools.
  • Focus on tools for guide RNA design (efficiency and specificity prediction).
  • Analysis of tools predicting CRISPR-Cas editing outcomes based on repair biases.
  • Evaluation of tools for analyzing sequencing data to assess editing efficiency.
  • Inclusion of relevant databases and repositories.

Main Results:

  • The review categorizes tools into three main groups: guide RNA design, prediction of editing outcomes, and analysis of editing results.
  • Tools for guide RNA design focus on predicting efficiency and specificity.
  • Emerging tools leverage repair biases to predict CRISPR-Cas editing outcomes.
  • Sequencing data analysis tools aid in evaluating editing success.
  • Associated repositories and databases are highlighted.

Conclusions:

  • A wide array of computational tools now supports CRISPR-Cas genome editing workflows.
  • These tools facilitate experimental design, prediction of outcomes, and analysis of results.
  • Addressing current limitations and challenges is crucial for advancing CRISPR-Cas technology and its applications.