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Using SLIM-Based IMS-IMS Together with Cryogenic Infrared Spectroscopy for Glycan Analysis
Priyanka Bansal1, Vasyl Yatsyna1,2, Ali H AbiKhodr1
1Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LCPM, Station 6, CH-1015 Lausanne, Switzerland.
Analytical Chemistry
|May 28, 2020
Summary
This study introduces a novel glycan analysis method combining ion mobility spectrometry (IMS) and cryogenic infrared spectroscopy. The technique accurately distinguishes glycan isomers, including linkage anomericity, overcoming limitations of traditional methods.
Area of Science:
- Analytical Chemistry
- Glycobiology
- Spectroscopy
Background:
- Glycan isomeric heterogeneity presents significant analytical challenges.
- Existing methods like ion mobility spectrometry-tandem mass spectrometry struggle with subtle isomer differentiation.
- Cryogenic infrared spectroscopy offers high structural sensitivity for glycan identification.
Purpose of the Study:
- To develop an advanced glycan analysis technique.
- To integrate ultrahigh-resolution ion mobility spectrometry-ion mobility spectrometry (IMS-IMS) with Structures for Lossless Ion Manipulation (SLIM) and cryogenic infrared spectroscopy.
- To enhance the characterization of glycan isomers, including linkage anomericity.
Main Methods:
- Design and implementation of a SLIM board with on-board traps for millibar-range collision-induced dissociation (CID).
- Characterization of the on-board CID process using a pentapeptide standard.
- Application of the integrated IMS-IMS and cryogenic infrared spectroscopy technique to human milk oligosaccharides.
Main Results:
- The on-board CID process was validated against a commercial tandem mass spectrometer.
- The technique successfully analyzed the mobility and vibrational spectra of CID fragments from human milk oligosaccharides.
- Specific glycan isomeric forms, including glycosidic linkage anomericity, were identified by comparing fragment drift times and IR spectra with reference compounds.
Conclusions:
- The combined SLIM-IMS-IMS and cryogenic infrared spectroscopy approach provides a powerful new tool for glycan analysis.
- This method overcomes limitations in distinguishing subtle glycan isomer differences.
- The technique demonstrates high potential for accurate and detailed glycan structure elucidation.
