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Updated: Dec 20, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

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Circular RNA circKIF4A Sponges miR-375/1231 to Promote Bladder Cancer Progression by Upregulating NOTCH2 Expression

Ying-Rui Shi1,2, Zheng Wu2, Kun Xiong1

  • 1Department of Urology, Xiangya Hospital, Central South University, Changsha, China.

Frontiers in Pharmacology
|May 28, 2020
PubMed

Insights

Circular RNAs (circRNAs) promote bladder cancer growth and metastasis. This study identifies circKIF4A as a key player, highlighting the circKIF4A-miR-375/1231-NOTCH2 axis in cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in cancer development and metastasis.
  • The specific roles of most circRNAs in bladder cancer progression are not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of circKIF4A (hsa_circ_0007255) in bladder cancer development and progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for circKIF4A expression detection.
  • Cell proliferation (CCK-8, colony-formation), migration, and invasion (Transwell) assays.
  • In vivo mouse xenograft models and molecular mechanism studies (luciferase reporter, RNA immunoprecipitation assays).

Main Results:

  • CircKIF4A was significantly upregulated in bladder cancer tissues and cell lines.
  • Knockdown of circKIF4A inhibited bladder cancer cell proliferation, colony formation, migration, and metastasis in vitro and in vivo.
  • CircKIF4A acts as a sponge for miR-375/1231, upregulating NOTCH2 and promoting bladder cancer progression via the competing endogenous RNA (ceRNA) mechanism.

Conclusions:

  • CircKIF4A plays a critical role in promoting bladder cancer progression.
  • The circKIF4A-miR-375/1231-NOTCH2 axis is a potential therapeutic target for bladder cancer treatment.
Keywords:
NOTCH2bladder cancercircKIF4Acircular RNAsmiR-375

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