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Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments.

Chin-Yi Cho1, Tzu-Hsiang Chiang1, Li-Hung Hsieh2

  • 1Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.

Frontiers in Cell and Developmental Biology
|May 28, 2020
PubMed
Summary

Researchers developed a new system for creating 3D microtissues, which better mimic natural tissues than traditional methods. This cost-effective platform enables faster, more consistent 3D microtissue fabrication for biomedical research.

Keywords:
arrayglomerulushanging dropmicrotissuepodocyte

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Traditional 2D cell cultures do not accurately represent the 3D tissue microenvironment.
  • Existing 3D microtissue fabrication methods are often complex, expensive, and time-consuming.
  • There is a need for accessible platforms to create physiologically relevant 3D models.

Purpose of the Study:

  • To develop an easy-to-use, cost-effective system for fabricating uniform 3D microtissues.
  • To create a tunable platform for controlling the 3D microenvironment and cellular composition of microtissues.
  • To generate heterogeneous microtissues for use as advanced biomedical research models.

Main Methods:

  • Development of a pressure-assisted network for droplet accumulation (PANDA) system.
  • Integration of a multichannel fluidic system with a hanging drop cell culture module.
  • Adjustment of seeding density to control microtissue size and cellular composition.

Main Results:

  • The PANDA system successfully produced uniform 3D microtissues.
  • The system allowed for control over microtissue size and cellular heterogeneity.
  • Highly consistent, glomerulus-like heterogeneous microtissues composed of podocytes and mesenchymal stem cells were fabricated.
  • The platform demonstrated rapid and economical fabrication of microtissues.

Conclusions:

  • The PANDA system offers a rapid, economical, and user-friendly platform for 3D microtissue fabrication.
  • This system enables the creation of tunable 3D microenvironments and cellular heterogeneity.
  • The developed microtissues serve as effective tissue-mimicking models for diverse biomedical research applications.