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Molecular weight of tumor necrosis factor determined by gel permeation chromatography alone or in combination with
T Yoshimura1, S Sone, S Maezawa
1Institute for Enzyme Research, School of Medicine, University of Tokushima, Japan.
Biochemistry International
|December 1, 1988
Abstract:
The molecular weight of human recombinant tumor necrosis factor was determined at neutral pH by gel permeation chromatography alone or in combination with low-angle laser light scattering. Mean values of 39,200 +/- 800 and 48,800 +/- 900, respectively, were obtained by the two analyses. The results resolve the apparent discrepancy in the reported values of the molecular weight of this cytokine, confirming that it exists as a trimer in neutral solution with a molecular weight of about 50,000.