Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Epoxy coating to prolong actuation time in degas-driven PDMS micropumps.

Soft matter·2026

Same author

The importance of choosing a proper validation strategy in predictive models. Part 2: Recipes for (avoiding) overfitting-A tutorial.

Analytica chimica acta·2026

Same author

Analyzing the Relationship between Solid-Phase Molecular Presentation and Cell Proliferation, Morphology and Secretion Using CellStudio.

ACS applied materials & interfaces·2025

Same author

Present and future of smart functional materials as actuators in microfluidic devices.

Lab on a chip·2025

Same author

A microfluidic device for passive separation of platelet-rich plasma from whole blood.

Lab on a chip·2025

Same author

Generation and Combination of Ionogel Microstructures Using the Vacuum-Driven Lithography Technique.

ACS applied materials & interfaces·2025

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Dec 20, 2025

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Optical Single Cell Resolution Cytotoxicity Biosensor Based on Single Cell Adhesion Dot Arrays.

Maite Garcia-Hernando^1,2, Alba Calatayud-Sanchez¹, Jaione Etxebarria-Elezgarai¹

¹BIOMICs-Microfluidics Research Group, Microfluidics & BIOMICS Cluster UPV/EHU, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain.

Analytical Chemistry

|May 29, 2020

Summary

A new single cell adhesion dot array (SCADA) method offers high-resolution cell viability testing. This digital imaging technique dynamically tracks cell death and toxicity, advancing high-throughput screening.

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Live-cell Imaging of Single-Cell Arrays LISCA - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays LISCA - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Related Experiment Videos

Last Updated: Dec 20, 2025

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Live-cell Imaging of Single-Cell Arrays LISCA - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays LISCA - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Area of Science:

Biotechnology
Cell Biology
Toxicology

Background:

Accurate cell viability testing is crucial for pharmaceutical, biomaterial, and environmental industries.
Existing methods struggle to dynamically monitor cell death, especially for compounds that promote cell adhesion.

Purpose of the Study:

To develop a high-resolution, easy-to-use cell viability assay.
To enable dynamic monitoring of cell death and cytotoxicity, even with compounds that affect cell adhesion.

Main Methods:

Developed "single cell adhesion dot arrays" (SCADA) using fibronectin dots for single-cell culture.
Utilized digital optical imaging for dynamic quantification of cell viability.
Applied SCADA to assess cytotoxicity of K2CrO4, HgSO4, and DMSO, incorporating trypan blue assay for specific compounds.

Main Results:

SCADA enabled dynamic monitoring of cell detachment rates for DMSO and K2CrO4 over 30 hours.
Successfully monitored cytotoxicity of HgSO4 using trypan blue assay directly on the SCADA surface.
Achieved single-cell resolution and results comparable to existing methods.

Conclusions:

SCADA provides a high-resolution, dynamic method for cell viability and cytotoxicity assessment.
This technique is adaptable for various toxic compounds, including those affecting cell adhesion.
Integration with microfluidics offers potential for high-throughput screening and broader applications.