Complete genome sequence of Tacaribe virus

Area of Science:

• Virology
• Molecular Biology
• Genomics

Background:

• Tacaribe virus (TCRV) is a prototype New World arenavirus, crucial for studying arenavirus biology despite not being a human pathogen.
• Existing TCRV genome sequences have discrepancies and are incomplete, hindering accurate molecular and genetic research.

Purpose of the Study:

• To provide the first complete genome sequence for the Tacaribe virus (TCRV) TRVL-11573 strain.
• To correct identified errors and complete the genome ends, including regulatory elements for RNA synthesis.

Main Methods:

• Next-generation sequencing to obtain the complete viral genome.
• Bioinformatic analysis to compare sequences and identify discrepancies.
• Phylogenetic analysis to compare with related strains.

Main Results:

• The first complete genome sequence for TCRV TRVL-11573 is presented, including completed 5' and 3' ends.
• Discrepancies in the S segment intergenic region and errors in the L segment 3' end were identified and corrected.
• Significant differences in the GP and NP proteins were noted, including a 12-amino-acid deletion in GP and a 4-amino-acid exchange in NP.

Conclusions:

• The corrected and complete TCRV genome sequence provides a reliable foundation for future research.
• The TRVL-11573 strain is nearly identical to a strain isolated in Florida in 2012.
• Accurate TCRV genomic data is essential for advancing arenavirus molecular and genetic studies.