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Updated: Dec 20, 2025
An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
Published on: March 9, 2021
Apoplastic Fluid Preparation from Arabidopsis thaliana Leaves Upon Interaction with a Nonadapted Powdery Mildew
Ryohei Thomas Nakano1,2, Nobuaki Ishihama3, Yiming Wang4,5
1Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany. nakano@mpipz.mpg.de.
Abstract:
Proteins in the extracellular space (apoplast) play a crucial role at the interface between plant cells and their proximal environment. Consequently, it is not surprising that plants actively control the apoplastic proteomic profile in response to biotic and abiotic cues. Comparative quantitative proteomics of plant apoplastic fluids is therefore of general interest in plant physiology. We here describe an efficient method to isolate apoplastic fluids from Arabidopsis thaliana leaves inoculated with a nonadapted powdery mildew pathogen.
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