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Updated: Dec 20, 2025

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS
Daisuke Takahashi1,2,3, Bin Li2, Takato Nakayama4
1Central Infrastructure Group: Genomics and Transcript Profiling, Max-Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
Abstract:
Shotgun proteomics allows for the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. However, this method is not fully applicable for highly hydrophobic proteins with multiple transmembrane domains. In order to solve this problem, we here describe a shotgun proteomics method using nano-LC-MS/MS for proteins in the plasma membrane and plasma membrane microdomain fractions. The results obtained are easily applicable to label-free protein semiquantification.

