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Quantitative Profiling of Protein Abundance and Phosphorylation State in Plant Tissues Using Tandem Mass Tags
Gaoyuan Song1, Christian Montes1, Justin W Walley2
1Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2020
Abstract:
Proteins produce or regulate nearly every component of cells. Thus, the ability to quantitatively determine the protein abundance and posttranslational modification (PTM) state is a critical aspect toward our understanding of biological processes. In this chapter, we describe methods to globally quantify protein abundance and phosphorylation state using isobaric labeling with tandem mass tags followed by phosphopeptide enrichment.

