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Updated: Dec 20, 2025

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
Published on: June 14, 2013
Analysis of exosomal lncRNA, miRNA and mRNA expression profiles and ceRNA network construction in endometriosis
Jingni Wu1, Hongyan Huang1, Wei Huang2,3
1Department of Obstetrics & Gynecology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Abstract:
Aim: To investigate exosomal RNAs (long noncoding RNAs (lncRNAs), microRNAs (miRNAs) and messenger RNAs (mRNAs)) profiling and their related networks in endometriosis (EMs). Materials & methods: RNA sequence was performed in exosomes from ovarian endometriomas (EC), eutopic endometria (EU) and normal endometria (Control) stromal cells. The bioinformatics algorithms evaluated competing endogenous RNA (ceRNA) networks. The top-ranked ceRNA networks were confirmed by RT-PCR. Results: Overlapped differentially expressed 938 lncRNAs, 39 miRNAs and 1449 mRNAs were identified. 13 co-expression modules and 61 ceRNA networks were constructed. Conclusion: This study for the first time shows exosomal RNA biomarkers and lncRNA-related networks in EMs, which reveals a novel molecular mechanism of EMs and provides new resources for EM diagnosis and treatment.
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Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

