Perchlorate bioreduction by anaerobic granular sludge immobilised with Fe-HA complex: Performance, extracellular...

An iron-humic acid (Fe-HA) complex was used as a redox mediator in perchlorate (ClO₄^-) bioreduction. Bioreduction performance, extracellular polymeric substances (EPS), and microbial community structure were comprehensively explored using different types of anaerobic granular sludge (AnGS) immobilised without the Fe-HA complex (AnGS_CON) and with the Fe-HA complex (AnGS_FH). The ClO₄^- was completely removed by AnGS_CON by day 20, while the ClO₄^- was completely removed by AnGS_FH by day 6. The AnGS immobilised with the Fe-HA complex significantly increased the ClO₄^- bioreduction. The acceleration of ClO₄^- bioreduction was also explained by the mixed liquor volatile suspended solids (MLVSS), MLVSS/mixed liquor suspended solids (MLSS), EPS composition, and microbial community structure. Compared with AnGS_CON, the MLVSS and MLVSS/MLSS of the AnGS_FH increased 1.4- and 1.2-fold, respectively. Humic substances in the EPS were stimulated by the Fe-HA complex. The microbial community structure analysis indicated that perchlorate and quinone reducing bacteria were enriched by the Fe-HA complex. Based on the analysis, the ClO₄^- bioreduction mechanism of the AnGS_FH was revealed because the Fe-HA complex in the AnGS increased the biomass concentration, biological activity, and redox-active mediator and shifted the microbial community structure.