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Epigenetic Modifications: An Unexplored Facet of Exogenous RNA Application in Plants
Athanasios Dalakouras1,2, Kalliope K Papadopoulou1
1Department of Biochemistry & Biotechnology, University of Thessaly, 41500 Larissa, Greece.
Abstract:
Exogenous RNA interference (exo-RNAi) is a powerful transgene-free tool in modern crop improvement and protection platforms. In exo-RNAi approaches, double-stranded RNAs (dsRNAs) or short-interfering RNAs (siRNAs) are externally applied in plants in order to selectively trigger degradation of target mRNAs. Yet, the applied dsRNAs may also trigger unintended epigenetic alterations and result in epigenetically modified plants, an issue that has not been sufficiently addressed and which merits more careful consideration.
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