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Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis

Dhara Malavia1, Neil A R Gow1, Jane Usher1

  • 1Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK.

Microorganisms
|May 30, 2020
PubMed

Insights

Advancements in molecular tools like CRISPR and RNA interference, alongside animal models, are crucial for studying fungal pathogens and combating antifungal drug resistance.

Area of Science:

  • Mycology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Pathogenic fungi pose a growing threat due to increasing antifungal drug resistance.
  • Understanding fungal pathogenicity and host interactions is critical for developing new treatments.

Purpose of the Study:

  • To review recent advances in molecular tools for studying fungal pathogens.
  • To discuss the utility of various animal models in fungal disease research.

Main Methods:

  • Review of molecular tools including CRISPR technologies, RNA interference, and transposon targeted mutagenesis.
  • Discussion of animal models such as zebrafish, silkworms (Galleria mellonella), and murine models.

Main Results:

  • CRISPR, RNAi, and transposon mutagenesis offer powerful methods for genetic manipulation of fungi.
  • Animal models provide valuable platforms for studying fungal infection dynamics and virulence.

Conclusions:

  • These molecular tools and animal models are essential for accelerating research into fungal infections and drug resistance.
  • Continued development and application of these technologies will aid in overcoming the challenge of fungal infectious diseases.
Keywords:
CRISPRRNAianimal modelsfungal pathogensgenetic tools

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