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Immune complex detection by immunofluorescence on polymorphonuclear leucocytes.

J W Steffelaar, F J Ten Kate, M Nap

Clinical and Experimental Immunology

|March 1, 1977

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Systemic lupus erythematosus (SLE) patients

Area of Science:

Immunology
Rheumatology
Cell Biology

Background:

Polymorphonuclear leucocytes (PMN) play a role in immune complex diseases.
Systemic lupus erythematosus (SLE) is characterized by autoantibodies and immune complex deposition.
Understanding PMN interactions with immune complexes is crucial for SLE pathogenesis.

Purpose of the Study:

To investigate the presence and origin of immunoglobulin (Ig) inclusions in PMN from SLE patients.
To assess the role of in vitro phagocytosis in PMN Ig uptake.
To determine if artificial immune complexes can be detected by PMN.

Main Methods:

Isolation of PMN from SLE patients and healthy donors.
Incubation of PMN with SLE sera and artificial immune complexes (HBsAg/anti-HBs).

Related Experiment Videos

Use of monoiodine acetic acid (MIAA) to inhibit in vitro phagocytosis.

Immunofluorescence technique to detect IgG, IgM, and HBsAg in PMN.

Main Results:

PMN from SLE patients' defibrinated blood showed high levels of Ig inclusions (up to 80%).
Inhibition of in vitro phagocytosis with MIAA reduced Ig inclusions in defibrinated blood PMN to levels seen in heparinized blood.
Normal PMN incubated with SLE sera showed Ig inclusions, which were abolished by MIAA.
Artificial immune complexes were detected by PMN, with detection varying by antigen/antibody ratio.

Conclusions:

Ig inclusions in PMN from heparinized SLE blood are primarily due to in vivo phagocytosis of circulating immune complexes.
In vitro phagocytosis experiments confirm the presence of immune complexes in SLE sera.
PMN can phagocytose artificial immune complexes, with efficiency dependent on the antigen-antibody ratio.