Related Experiment Video
Updated: Dec 20, 2025
07:07
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
11.7K
Neural Regeneration/Remodeling in Engineered Coronal Pulp Tissue in the Rat Molar
Phyo Pyai Sone1, Tomoatsu Kaneko1, Su Yee Myo Zaw1
1Department of Pulp Biology and Endodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Journal of Endodontics
|May 30, 2020
Summary
This study shows that bone marrow mesenchymal stem cells promote nerve regeneration in rat teeth. This tissue engineering approach successfully restored nerve fibers and growth factor expression in pulpotomized molars.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Regenerative Medicine
- Neuroscience
Background:
- Pulp tissue regeneration is crucial for maintaining tooth vitality.
- Understanding reinnervation processes is key to successful pulp repair.
- Mesenchymal stem cells offer potential for regenerative therapies.
Purpose of the Study:
- To investigate nerve regeneration during coronal pulp tissue engineering.
- To evaluate the role of rat bone marrow mesenchymal stem cells (rBMSCs) in reinnervation.
- To analyze neuronal marker expression and gene activity in a rat pulpotomy model.
Main Methods:
- Pulpotomy was performed on Wistar rat molars.
- Biodegradable scaffolds with rBMSCs were implanted.
- Histologic analysis and immunoperoxidase staining (PGP 9.5, CGRP, SP) were conducted.
- Real-time PCR measured nerve growth factor (NGF) and GAP-43 mRNA levels.
Main Results:
- Histology showed dentin bridge formation and scaffold resorption by day 14.
- Nerve fiber density (PGP 9.5, CGRP) increased significantly over 14 days.
- Substance P (SP) nerve fibers peaked at day 7, returning to normal by day 14.
- NGF mRNA increased, while GAP-43 mRNA peaked early and then declined.
Conclusions:
- Coronal pulp tissue engineering with rBMSCs facilitates nerve regeneration.
- The study demonstrates remodeling of SP and CGRP nerve fibers.
- Increased NGF and GAP-43 mRNA expression supports the regenerative process.
