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Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

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Coding-Complete Genome Sequences of Two SARS-CoV-2 Isolates from Egypt.

Ahmed Kandeil¹, Ahmed Mostafa¹, Rabeh El-Shesheny¹

¹Center of Scientiﬁc Excellence for Inﬂuenza Viruses, National Research Centre, Giza, Egypt.

Microbiology Resource Announcements

|May 30, 2020

Summary

Researchers sequenced two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains from Egypt. Analysis revealed unique mutations differentiating these Egyptian SARS-CoV-2 variants from the original Wuhan strain.

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Area of Science:

Virology
Genomics
Epidemiology

Background:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global health concern.
Understanding viral genetic diversity is crucial for public health.
Limited genomic data exists for SARS-CoV-2 strains in Egypt.

Purpose of the Study:

To report the complete genome sequences of two SARS-CoV-2 isolates from Egypt.
To identify genetic variations in Egyptian SARS-CoV-2 strains.

Main Methods:

Whole-genome sequencing of SARS-CoV-2 isolates.
Bioinformatic analysis of obtained genome sequences.
Comparison with reference SARS-CoV-2 genome (2019-nCoV WHU01).

Main Results:

Complete genome sequences for two Egyptian SARS-CoV-2 isolates were obtained.
Sequence analysis identified specific mutations distinguishing these isolates.
The Egyptian strains exhibited genetic differences compared to the reference strain.

Conclusions:

The study provides valuable genomic data for SARS-CoV-2 strains circulating in Egypt.
Identified mutations may influence viral characteristics or transmission.
Further research is needed to understand the implications of these genetic variations.