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Novel Process for 3D Printing Decellularized Matrices
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Biocompatible PEGDA Resin for 3D Printing.

Chandler Warr1, Jonard Corpuz Valdoz2, Bryce P Bickham3

  • 1Chemical Engineering Department, Brigham Young University, Provo, Utah, USA 84602.

ACS Applied Bio Materials
|May 30, 2020
PubMed
Summary
This summary is machine-generated.

A new non-cytotoxic 3D printing resin, avobenzone-PEGDA (A-PEGDA), is developed for high-resolution cell-based applications. This resin, compatible with custom 3D printers, shows excellent biocompatibility and supports cell spheroid formation and migration.

Keywords:
3D printingbiocompatibilitycytotoxicitymicrofluidicsresinspheroid

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Area of Science:

  • Biomaterials Science
  • Microfluidics
  • Cell Biology

Background:

  • Custom high-resolution 3D printing enables advanced microfluidic devices.
  • Biocompatible resins are crucial for cell-based applications in 3D printed structures.
  • Traditional resins may exhibit cytotoxicity or require post-processing for cell compatibility.

Purpose of the Study:

  • To develop and evaluate a non-cytotoxic resin for high-resolution 3D printing.
  • To assess the biocompatibility and cell adhesion properties of the novel resin.
  • To demonstrate the utility of the resin in cell-based assays, including spheroid formation and migration.

Main Methods:

  • A novel poly(ethylene glycol) diacrylate (PEGDA) resin incorporating avobenzone as a UV absorber (A-PEGDA) was synthesized.
  • Cytotoxicity and cell adhesion were evaluated for both A-PEGDA and 2-nitrophenyl phenyl sulfide-PEGDA (NPS-PEGDA) resins.
  • 3D printed structures were assessed in their as-printed state and after plasma treatment for cell adhesion.
  • Spheroid formation and cell migration assays were performed using A-PEGDA in 3D printed wells.

Main Results:

  • Avobenzone-PEGDA (A-PEGDA) resin demonstrated effective non-cytotoxicity in its as-printed state.
  • NPS-PEGDA resin required a 12-hour ethanol wash to achieve non-cytotoxicity.
  • As-printed resins showed limited cell adhesion, which significantly increased after plasma treatment.
  • A-PEGDA facilitated spheroid formation in ultra-low adhesion 3D printed wells and supported cell migration.

Conclusions:

  • A-PEGDA is a promising non-cytotoxic resin for high-resolution 3D printing in cell-based applications.
  • Plasma treatment enhances cell adhesion on 3D printed surfaces, expanding their utility.
  • The developed resin enables advanced cell culture models, including spheroid formation and migration studies within 3D microfluidic structures.