Related Experiment Video
Updated: Dec 20, 2025
07:35
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
Published on: August 3, 2017
6.9K
Correction to: Enzymatic studies on aromatic prenyltransferases
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. tmori@mol.f.u-tokyo.ac.jp.
Journal of Natural Medicines
|May 30, 2020
Summary
This study explores aromatic prenyltransferases, enzymes crucial for synthesizing plant compounds. Researchers investigated their enzymatic mechanisms to understand natural product biosynthesis.
Area of Science:
- Biochemistry
- Enzymology
- Natural Product Synthesis
Context:
- Aromatic prenyltransferases are key enzymes in the biosynthesis of diverse plant secondary metabolites.
- Understanding these enzymes is vital for elucidating complex metabolic pathways in plants.
Purpose:
- To investigate the enzymatic properties and mechanisms of aromatic prenyltransferases.
- To characterize the substrate specificity and catalytic activity of these enzymes.
Summary:
- The research details enzymatic studies on aromatic prenyltransferases, focusing on their catalytic functions.
- Characterization of these enzymes provides insights into the formation of prenylated aromatic compounds.
Impact:
- This work contributes to the fundamental understanding of plant biochemistry and natural product biosynthesis.
- Findings can inform future efforts in metabolic engineering and drug discovery from plant sources.
More Related Videos
Related Concept Videos
01:16Electrophilic Aromatic Substitution: Overview
13.3K
In an electrophilic aromatic substitution reaction, an electrophile substitutes for a hydrogen of an aromatic compound.
13.3K
01:25Aromatic Compounds: Overview
13.1K
In general, the term ‘aromatic’ indicates a pleasant smell or fragrance from fresh flowers, freshly prepared coffee, etc. In the early history of organic chemistry, many benzene derivatives were isolated from the pleasant odor oils of the plants. For example, vanillin was isolated from the oil of vanilla, methyl salicylate from the oil of wintergreen, and cinnamaldehyde from the oil of cinnamon. They all had a pleasant odor; hence the name aromatic was given.
In 1825, Faraday isolated...
In 1825, Faraday isolated...
13.1K
01:14π Electron Effects on Chemical Shift: Aromatic and Antiaromatic Compounds
1.7K
In aromatic compounds, such as benzene, the circulation of (4n + 2) π-electrons sets up a diamagnetic or diatropic ring current around the perimeter of the molecule. This current induces a magnetic field that opposes the external field inside the ring and reinforces it on the outside. The protons in benzene are deshielded and exhibit high chemical shifts in the range 6.5–8.5 ppm. The shielding effect at the center of the ring is evident in complex aromatic molecules, such as...
1.7K
01:19Allosteric Proteins-ATCase
6.4K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.4K
01:11ortho–para-Directing Activators: –CH3, –OH, –NH2, –OCH3
7.1K
All ortho–para directors, excluding halogens, are activating groups. These groups donate electrons to the ring, making the ring carbons electron-rich. Consequently, the reactivity of the aromatic ring towards electrophilic substitution increases. For instance, the nitration of anisole is about 10,000 times faster than the nitration of benzene. The electron-donating effect of the methoxy group in anisole activates the ortho and para positions on the ring and stabilizes the corresponding...
7.1K
01:26Photochemical Electrocyclic Reactions: Stereochemistry
2.1K
The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
Selection Rules: Photochemical Activation
2.1K