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An artificial protein-probe hybrid as a responsive probe for ratiometric detection and imaging of hydrogen peroxide
Xing Zhang1, Youxin Fu2, Guangren Qian3
1School of Environmental Science and Chemical Engineering, Shanghai University, Shanghai 200444, China. grqian@shu.edu.cn and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia. gordonxu@uq.edu.au.
Abstract:
An artificial protein-probe hybrid, Cm-Np-B@BSA, was prepared via host-guest interactions between hydrogen peroxide (H2O2)-responsive Cm-Np-B molecule and bovine serum albumin (BSA). The Cm-Np-B@BSA probe exhibited high sensitivity and selectivity towards H2O2 under physiological conditions and had excellent biocompatibility, allowing for sensitive ratiometric detection and imaging of endogenous H2O2 in live cells.

