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NR2F2-AS1 accelerates cell proliferation through regulating miR-4429/MBD1 axis in cervical cancer
Dan Liu1, Kejin Huang2, Tiaojiao Wang1
1The Seventh Department of the Internal Medicine, Harbin Medical University Cancer Hospital, Harbin 150081, Heilongjiang, China.
Bioscience Reports
|May 30, 2020
Summary
Long noncoding RNA NR2F2 antisense RNA 1 (NR2F2-AS1) promotes cervical cancer progression by regulating the miR-4429/MBD1 axis. This study reveals NR2F2-AS1 as a potential therapeutic target for cervical cancer.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Cervical cancer is a frequent malignancy in women.
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
- The specific role and mechanism of lncRNA NR2F2 antisense RNA 1 (NR2F2-AS1) in cervical cancer remain unclear.
Purpose of the Study:
- To investigate the expression and function of NR2F2-AS1 in cervical cancer.
- To elucidate the molecular mechanism underlying NR2F2-AS1's role in cervical cancer progression.
- To explore the potential of NR2F2-AS1 as a therapeutic target.
Main Methods:
- Quantitative real-time PCR to assess NR2F2-AS1 expression in cervical cancer tissues and cells.
- Cell proliferation, migration, invasion, and apoptosis assays to evaluate NR2F2-AS1 function.
- Molecular sponge assays and Western blotting to investigate the interaction between NR2F2-AS1, miR-4429, and MBD1.
- Rescue experiments to validate the regulatory pathway.
Main Results:
- NR2F2-AS1 expression was significantly upregulated in cervical cancer tissues and cells, particularly in advanced stages.
- NR2F2-AS1 promoted cervical cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while inhibiting apoptosis.
- NR2F2-AS1 functioned as a molecular sponge for miR-4429, and methyl-CpG-binding domain protein 1 (MBD1) was a downstream target of miR-4429.
- A negative correlation was observed between miR-4429 and NR2F2-AS1 or MBD1 expression in tumor tissues.
- MBD1 overexpression partially rescued the effects of NR2F2-AS1 knockdown on cervical cancer progression.
Conclusions:
- NR2F2-AS1 plays a carcinogenic role in cervical cancer by promoting tumor progression.
- The NR2F2-AS1/miR-4429/MBD1 axis is a key regulatory pathway in cervical cancer.
- NR2F2-AS1 represents a promising therapeutic target for cervical cancer treatment.
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