Study on polyethylene glycol cross-linker in peptide-conjugated antibody on efficiency of cell capture and release

Cell separation is important in cell therapy and disease diagnosis. Therefore, various cell separation methods have been studied, but cellular damage and the need for pretreatment remain substantial problems. Recently, in the diagnostic field, the detachment and recovery of antibody-captured cells was actively studied to obtain more detailed information on cancer cells. Previously, we have developed a highly efficient cell separation method using microfibers. In the present study, the efficiency of cell capture and release was examined by controlling the molecular mobility of an immobilized antibody to efficiently detect cells with low expression of a marker molecule. We found that improvement in molecular mobility of antibodies enhances cell capture efficiency but decreases the detachment effectiveness of the captured cells. Therefore, the molecular mobility of antibodies can be utilized to control cell capture and release according to the level of expression of the marker molecule.