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Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

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Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
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The Calvin Benson Cycle01:46

The Calvin Benson Cycle

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Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
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Bioremediation00:46

Bioremediation

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Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
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Carbon Dioxide Transport in the Blood01:19

Carbon Dioxide Transport in the Blood

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Carbon dioxide (CO2) transport in the blood is critical to human physiology. On average, our body cells produce around 200 mL of CO2 per minute, precisely the quantity expelled by the lungs. This process involves the transportation of CO2 from the tissue cells to the lungs in three primary forms.
Forms of CO2 Transport
1. Dissolved in plasma: A small percentage (7-10%) of CO2 is transported and dissolved directly in the plasma.
2. Carbaminohemoglobin: Just over 20% of CO2 is chemically bound to...
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Anoxygenic Photosynthesis01:30

Anoxygenic Photosynthesis

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Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
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C4 Pathway and CAM01:27

C4 Pathway and CAM

48.4K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
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Related Experiment Video

Updated: Dec 20, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

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Biological CO2 fixation in up-flow reactors via exogenous H2 addition.

P G Kougias1, P Tsapekos2, L Treu3

  • 1Hellenic Agricultural Organisation Demeter, Institute of Animal Science, 58100 Paralimni, Greece.

Journal of Biotechnology
|May 30, 2020
PubMed
Summary

Gas fermentation using CO2 and H2 is a promising method for reducing greenhouse gas emissions. Packing material in up-flow reactors significantly enhances methane production by improving gas utilization.

Keywords:
Anaerobic digestionCO(2)fixationHomoacetogenesisHydrogenotrophic methanogenesisPacked column reactors

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Area of Science:

  • Biotechnology
  • Environmental Science
  • Chemical Engineering

Background:

  • Greenhouse gas emissions reduction is a global priority.
  • Gas fermentation offers a sustainable route for producing biofuels and chemicals.
  • Biological conversion of carbon dioxide (CO2) and hydrogen (H2) is crucial for carbon capture and recycling.

Purpose of the Study:

  • To evaluate the performance of two up-flow reactors for CO2 and H2 assimilation.
  • To investigate the impact of reactor design on gas transfer and utilization.
  • To identify key microbial communities involved in the biomethanation process.

Main Methods:

  • Comparative analysis of two up-flow reactor configurations.
  • Process monitoring of gas-liquid hydrogen transfer and gas utilization.
  • High-throughput 16S rRNA sequencing for microbial community analysis.

Main Results:

  • Reactor design significantly impacts gas-liquid H2 transfer.
  • A reactor packed with Raschig rings achieved 81% CH4 content with enhanced gas utilization.
  • Absence of packing material resulted in limited biomethanation, underscoring the role of packing.

Conclusions:

  • Packing material, specifically Raschig rings, plays a vital role in enhancing the performance of up-flow reactors for gas fermentation.
  • Optimized reactor design is key to efficient CO2 and H2 assimilation for methane production.
  • Methanothermobacter methanogens were identified as the dominant microbial species in the reactor.