Synthesis and characterization for a highly selective bis(salamo)-based chemical sensor and imaging in living cell

A new sensor H₅L for continuous identification of Cu²⁺, Al³⁺ and lysine was synthesized by Schiff base reactions. The sensor could specifically recognized Cu²⁺ in the EtOH/H₂O (1:1 v/v) solution by UV-vis spectra, and the binding constant with Cu²⁺ can reach 10¹¹ M^-1, meanwhile, it was found by the naked-eye that the color of the solution was changed from colorless to yellow. The copper complex L-Cu²⁺ formed by the sensor H₅L and Cu²⁺ could further recognize Al³⁺ and lysine in the fluorescence spectra. The LOD values of the three objects were 2.67 × 10^-8, 1.96 × 10^-8 and 5.59 × 10^-9 M, respectively. In addition, fluorescence intracellular images of Al³⁺ and lysine were performed and obtained satisfactory results.